Professor
NSERC Industrial Senior Chair
Research Interests:
Forest Sciences Centre 4043
2424 Main Mall
Vancouver, BC V6T 1Z4
Canada
work phone: 604-822-9741

Areas of research include:

  • Application of enzymes in enhancing pulp and fibre properties
  • Fibre modification and bleach boosting pulps
  • Bioconversion of lignocellulosic residues to ethanol
  • Microbiology of waste water treatment
  • Application of fungi to upgrading and modification of forest products, pulp and paper and waste streams

Projects

Senior Chair in Forest Products Biotechnology

NSERC/Forintek/ Weyerhaeuser Canada

Substrate and enzyme factors that influence fiber modification

NSERC

Assessing factors that limit the enzymatic hydrolysis of softwood derived cellulose

NSERC

Bioconversion of softwoods to ethanol

NRC

Full characterization of western red cedar wood oil and the isolation and testing of tropolenes from the oil

BCASI/FRBC

Enzymatic and microbial treatment of pulp mill white waters

NCE

Use of immunoassays to monitor in-mill and waste water concentrations of hydrophobic, organic material

NCE

The use of enzymes to elucidate the structure and to modify native and recycled paper

NSERC/CFS/Industry

IEA bioconversion task, Biotechnology for the conversion of lignocellulosics

IEA

Life cycle analyses of biomass-to-ethanol

NCE

Supply of pretreated biomass for surface characterization and enzymatic digestion studies

NREL

Bioprocessing Centre for Sustainable Fuels

CFI

Development of a technically and economically viable pre-treatment and enzymatic process for the conversion of softwood residues to ethanol

NSERC/BIOCAP

Development of value-added bioproducts from the bioconversion of lignocellulosics

NSERC/BIOCAP

Development of biorefinery technologies for conversion of softwood residues to ethanol and co-products

NSERC

Participation in Biomass Consortium on Applied Fundamentals

NRC


Professional Affiliations

American Chemical Society ACS
American Society of Microbiology
Society of Industrial Microbiology

Awards

LifeSciences BC Leadership Award , 2009
Fellow of the Royal Society of Canada , 2007
Advances in Science and Technology Award, US Dept. of Agriculture, Peoria Illinois , 2000
IUFRO Scientific Achievement (given out every 5 years) , 1999
Charles D. Scott Awards for Scientific/Technical Contributions to Biotechnology for the Production of Fuels and Chemicals , 1998

Current Graduate Students

William Cadham, MSc
Under what circumstances can biofuels be used to maximize biomass utilization value? an analysis of leading biofuel jurisdictions based on their policy, market and technology characteristics
Keith Gourlay, PhD
Cellulose Amorphogenesis
Jinguang Hu, PhD
Assessing the interactions between cellulase and xylanase components to determine an optimum combination to achieve efficient hydrolysis of pretreated lignocellulosic substrates
Ling (Rachel) Li, MSc
Development of Biofuels Policy in China
Amadeus Pribowo, PhD
Enzyme recycling
Emily Dou, PhD
Pulp properties that influence the ability of enzymes to facilitate the conversion of kraft to dissolving grade pulps
Seena Kumar, MSc
Increasing the ethanol titer in fermentations of softwood derived sugar streams
Tony Mok, MSc
Rui Zhai, PhD
Na Zhong, PhD
High consistency hydrolysis and fermentation of lignocellulosic substrates for improved ethanol production
Mandy Lin, MSc
Timo van der Zwan, MSc

Post Doctoral Fellows and Research Assistants

Arantes, Valdeir
Chandra, Richard
Karatzos, Sergios
Piddocke, Maya
Van Dyk, Susan

Current Courses

Winter 2014

WOOD249 Contemporary Topics in Forestry and Wood Products Sections

Seminars on current forestry and wood products topics.

Selected Publications

Teresita Marzialetti, Juan Pablo Salazar, Constanza Ocampos, Richard Chandra, Pablo Chung, Jack Saddler, Carolina Parra (2014). Second-generation ethanol in Chile: optimisation of the autohydrolysis of Eucalyptus globulus – Biomass Conversion and Biorefinery DOI 10.1007/s13399-014-0114-x
Hu J, Arantes V, Pribowo A & Saddler JN (2013). The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific – Biotechnology for Biofuels 6: 112
Gourlay, K., Hu, J., Arantes, V., Andberg, M., Saloheimo, M., Penttilä, M., Saddler, J., (2013). Swollenin Aids in the Amorphogenesis Step during the Enzymatic Hydrolysis of Pretreated Biomass – Bioresource Technology (2013), 142, 498-503
Panagiotopoulos, I.A., Chandra, R.P., Saddler, J.N. (2013). A two stage pretreatment approach to maximise sugar yield to enhance reactive lignin recovery from poplar wood chips. – Bioresource Technology, 130, 570–577
Kapu, S.L., Piddocke, M., Saddler, J.N. (2013). High gravity and high cell density mitigate some of the fermentation inhibitory effects of softwood hydrolysates – AMB Express (2013). 3:15 doi: 10.1186/2191-0855-3-15
Saddler, J.N., Kumar, L. (2013). Special Issue from the NSERC Bioconversion network workshop: pretreatment and fractionation of biomass for biorefinery/biofuels – Biotechnology for Biofuels 2013, 6:17
Pan XJ, Saddler J.N. (2013). Effect of replacing Polyol by organosolv and kraft lignin on the property and structure of rigid polyurethane foam – Biotechnology for Biofuels 2013, 6:12
Pribowo,A.Y., Hu, J., Arantes, V., Saddler, J.N. (2013). The development and use of an ELISA-based method to follow the distrobution of cellulase monocomponents during the hydrolysis of pretreated corn stover – Biotechnology for Biofuels (2013) 6:80
Stephen, J.D., Mabee, W.E., Saddler, J.N. (2013). Lignocellulosic ethanol production from woody biomass: The impact of facility siting on competitiveness. – Energy Policy (2013)
Kumar Linoj, Saddler JN, Arantes Valdeir, Chandra Richard (2012). The lignin present in steam pretreated softwood binds enzymes and limites cellulose accessibility – -Bioresource Technology Volume 103, Issue 1, Page 201-208.
Fei Shen , Jinguang Hu, Yuehua Zhong , Michael L.Y. Liu , Jack N. Saddler Ronghou Liu (2012). Ethanol production from steam-pretreated sweet sorghum bagasse with high substrate consistency enzymatic hydrolysis – -Biomass and Bioenergy 2012 ( 1-8)
Luis F. Del Rio, Richard P. Chandra, Jack N. Saddler (2012). The ease of Enzymatic hydrolysis of Organosolv-pretreated softwoods – -Bioresource Technology 107(2012) 235-242
Saddler, J.N. Del Rio, Luis F. Chandra, Richard (2012). Fibre size does not appear to influcence the ease of enzymatic hydrolysis of organosolv-pretreated softwoods. – -Bioresource Technology 107(2012) 235-242.
Olsen C, Arantes V, Saddler J.N. (2012). The use of predictive models to optimize sugar recovery obtained after the steam pretreatment of softwoods – Biofuels, Bioproducts & Biorefining, 6:534-548.
Saddler JN, Gourlay K, Arantes V. (2012). Use of substructure-specific carbohydrate binding modules to track changes in cellulose accessibility and surface morphology during the amorphogenesis step of enzymatic hydrolysis. – -Biotechnology for Biofuels, 5:51.
Saddler JN, Kumar L, Arantes V. Chandra R (2012). The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. – -Bioresource Technology, 103:201-208
Saddler JN, Arantes V, Pribowo A (2012). The absorption and enzyme activity profiles of specific Trichoderma reesei Cellulase/Xylanase components when hydrolyzing steam pretreated corn stover. – Enzyme and Microbial Technology, 50:195-203
Kumar, L., Tooyserkani, Z., Sokhansanj, S., Saddler, J.N. (2012). Does densification influence the steam pretreatment and enzymatic hydrolysis of softwoods to sugars? – Bioresource Technology (2012), 6:15, 121, 190-198
Saddler, J.N. Mabee, Warren E. Simms, Ralph and Taylor, Michael. (2012). The Biorefining Story: Progress in the Commercialization of Biomass-to-Ethanol – T. Schlichter and L. Montes ( eds.), Forests in Development: A Vital Balance, DOI 10.1007/978-94-007-2576-8_4, Springer Science +Business Media B.V. 2012 page 39-51.
Chandra, RP, Au-Yeung K, Chanis C, Roos AA, Mabee W, Chung PA, Ghatora S, and Saddler, J. N. (2011). The Influence of Pretreatment and Enzyme Loading on the Effectiveness of Batch and Fed-Batch Hydrolysis of Corn Stover – -Biotechnology Progress 2011. Volume 27 Issue 1, pages 77-85
Del Rio L, Chandra R.P, Saddler J.N. (2011). The Effects of Increasing Swelling and Anionic Charges on the Enzymatic Hydrolysis of Organosolv-preatreated Softwoods at Low Enzyme Loadings. – –Biotechnology and Bioengineering Volume 108, issue 7, page 1549-1558
Nakagame S, Chandra R.P., Saddler J.N (2011). The influence of lignin on the enzymatic hydrolysis of pretreated biomass substrates. – -Sustainable Production of Fuels, Chemicals, and Fibers from Forest Biomass. American Chemical Society. Chapter 6, page 145-167 ACS Symposium Series Volume 1067.
Nakagame S, Chandra R.P., Kadla J.F., Saddler J.N. (2011). The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on teh enzymatic hydrolysis of cellulose. – -Bioresource Technology ( 2011) 102, 4507-4517
Arantes, V. and Saddler, J.N. (2011). Cellulose accessibility limits the effectiveness of minimum cellulase laoding on the efficient hydrolysis of pretreated lignocellulosic substrates – -Biotechnology for Biofuels 4:3
Nakagame, S., Chandra, R., Kadla, J.F., Saddler, J. (2011). Enhancing the enzymatic hydrolysis of lignocellulosic biomass by increasing the carboxylic acid content of the associated lignin – – Biotechn Bioeng 108, 538-548
Hu Jinguang, Arantes Valdeir, Saddler JN (2011). The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistirc effect? – -Biotechnology for Biofuels 2011 4:36
Kumar Linoj, Chandra Richard, Saddler JN (2011). Influence of steam pretreatment severity on post-treatments used to enhance the enzymatic hydrolysis of pretreated softwoods at low enzyme loadings. – -Biotechnology and Bioengineering. Vol.108(10): 2300-2311.
Mabee Waren, Saddler JN, Stephen James D. (2011). Will Second-Generation Ethanol be able to compete with First-Generation Ethanol? Opportunities for cost reduction – DOI: 10.1002/bbb.331; Biofuels, Bioprod. Bioref. (2011)
Shen, F., Kumar, L., Hu, J., Saddler, J. N. (2011). Evaluation of hemicellulose removal by Xylanase and delignification on SHF and SSF for bioethanol production with steam-pretreated substrates. – -Bioresource Technology, doi: 10.1016/j.biortech.2011.07.028
Arantes, V. and Saddler, J.N. (2010). Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis – -Biotechnology for Biofuels, 3:4, 1-11
Stephen, James D., Mabee, Warren E., Saddler, Jack N. (2010). Biomass logistics as a determinant of second-generation biofuel facility scale, location and technology selection – -Biofpr, Biofuels Bioproducts & Biorefining 2010, DOI:10.1002/bbb.239;4:503-518 (2010)
Kumar, L., Chandra, R. P., Chung, P.A., Saddler, J. N. (2010). Can the same steam pretreatment conditions be used for most softwoods to achieve good, enzymatic hydrolysis and sugar yields? – -Bioresource Technology 2010; 101:7827-7833
Mabee, W., Sadder, J. N. (2010). Bioethanol from lignocellulosics: Status and perspectives in Canada – -Bioresource Technology2010; 101:4806-4813
Nakagame, S., Chandra, R. P. and Saddler, J. N. (2010). The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis – -Biotechnol Bioeng. 2010 Apr 1;105(5):871-9
Del-Rio, L.F., Chandra, R. P., and Saddler, J. N. (2010). The effect of varying organosolv pretreatment chemicals on the physiochemical properties and cellulolytic hydrolysis of mountain pine beetle killed lodgepole pine – Applied Biochemistry and Biotechnology 2010; 161:1-21
Ackom, E., Mabee, W., Saddler, J. N. (2010). Industrial Sustainability of Competing Wood Energy Options in Canada – Applied Biochemistry Biotechnology 2010 ( In Press)
Sims, R.E.H., Mabee, W., Saddler, J.N. and Taylor, M. (2010). An overview of second generation biofuel technologies – -Bioresource Technology Volume 101 issue 6, page 1570-1580
Tu, M.B. and Saddler, J.N. (2010). Potential enzyme cost reduction with the addition of surfactant during the hydrolysis of pretreated softwood – -Applied Biochemistry and Biotechnology 161 (1-8): 274-287
Del Rio, L and Saddler, J.N. (2009). The Effect of Varying Organosolv Pretreatment Chemicals on the Physicochemical Properties and Cellulolytic Hydrolysis of Mountain Pine Beetle – killed Lodgepole Pine – -Applied Biochemistry and Biotechnology Part A: Enzyme Engineering and Biotechnology, Article 8786
Tu, M.B., Pan, X.J., Saddler, J.N. (2009). Adsorption of Cellulase on Cellulolytic Enzyme Lignin from Lodgepole Pine – -Journal of Agricultural and Food Chemistry 57 (17):7771–7778
Tu, M.B., Zhang, X., Paice, M., McFarlane, P.; Saddler, J.N. (2009). The Potential of Enzyme Recycling during the Hydrolysis of a Mixed Softwood Feedstock – -Bioresource Technology 100(24):6407-6415
Tu, M.B., Zhang, X., Paice, M., McFarlane, P.; Saddler, J.N. (2009). Effect of Surfactants on Separate Hydrolysis Fermentation and Simultaneous Saccharification Fermentation of Pretreated Lodgepole Pine – -Biotechnology Progress 25(4):1122-1129
Bura, R., Chandra, R. and Saddler, J.N. (2009). The influence of xylan on the enzymatic hydrolysis of steam pretreated corn stover and hybrid poplar – -Biotechnology Progress 25(2):315-322
Chandra, R.P., Ewanick, S.M., Chung, P.A., Au-Yeung, K., Del Rio, L.. Mabee, W. & Saddler, J.N. (2009). Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates – -Biotechnol Lett 31:1217-1222
Zhang, X., Qin, W., Paice, M.G. and Saddler, J.N. (2009). High consistency enzymatic hydrolysis of hardwood substrates – -Bioresource Technology 100:5890-5897
Keating, J., Johansson, C.I., Saddler, J.N. and Beatson, R.P. (2008). Kinetics of hydrogen peroxide brightening of western red cedar (Thuja plicata Donn) CTMP – -Journal of Wood Chemistry and Technology, 28(2):153-166
Pan, X-J, Xie, D., Yu, R.W. and Saddler, J.N. (2008). The bioconversion of mountain pine beetle-killed lodgepole pine to fuel ethanol using the organosolv process – –Biotechnology and Bioengineeering, 101(1):39-48
Chandra, R.P., Ewanick, S.M., Hsseih, C. and Saddler, J.N. (2008). The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis part 1: A modified Somons’ staining procedure – -Botechnology Progress, 24(5):1178-1185
Munoz, C., Menoca, R., Baeza, j., Berlin, A., Saddler, J.N. and Freer, J. (2007). Bioethanol production from bi-organosolv pulps of Pinus radiata and Acacia dealbata – -Journal of Chemical Technology & Biotechnology, 82(8):767-774
Pan, X.J., Xie, D., Kang, Y., Yoon, S.L. and Saddler, J.N. (2007). Effect of organosolv ethanol pretreatment variables on physical characteristics of hybrid poplar substrates – -Applied Biochemistry and Biotechnology 136-140:367-378
Chandra, R.P., Bura, R., Mabee, W., Berlin, A., Pan, X. an Saddler, J.N. (2007). Substrate Pretreatment: The Key to Effective Enzymatic Hydrolysis of Lignocellulosics? – -Special Volume of Advances in Biochemical Engineering/Biotechnology DOI 10.1007/10_2007_064
Tu, Maobing, Chandra, R. and Saddler, J.N. (2007). Evaluating the Distribution of Cellulases and the Recycling of Free Cellulases during the Hydrolysis of Lignocellulosic Subtrates – -Biotechnology Progress Publlication 23(2):398-406
Chandra, R.P., Esteghalilan, A.R., and Saddler, J.N. (2007). Assessing Substrate Accessibility to Enzymatic Accessibility of Enzymatic Hydrolysis by Celulases – -Book Chapter in American Chemical Society Book
Chandra, R.P., Beatson, R.P. and Saddler, J.N. (2007). Treatment of Douglas-fir Heartwood TMP with Laccases: Effect of Treatment Conditions on Peroxide Bleaching – -Journal of Wood Chemistry and Technology 27(2):73-82
Ewanick, S., Bura, R. and Saddler, J.N. (2007). Acid-catalyzed steam pretreatment of Lodgepole pine and subsequent enzymatic hydrolysis and fermentation to ethanol. – -Biotechnology and Bioengineering 98(4):737-746
Adamowicz, V., Boyda, A. and McFarlane, P.N. (2007): (2007). Global Wood Supply. Report #02 in the Series on “Drivers of Change in Canada’s Forests and Forest Sector”, prepared for the Forest Futures Project of the SFM Network, University of Alberta. 16 pp. November. – –SFM Network, University of Alberta
Mabee, W.E., Fraser, E.D.G., McFarlane, P.N. and Saddler, J.N. (2007). Canadian biomass reserves for biorefining. – –Applied Biochemistry and Biotechnology 129 (1-3) 22-40.
Tu, M., Chandra, R.P. and Saddler, J.N. (2007). Recycling Cellulases during the Hydrolysis of Steam Exploded and Ethanol Pretreated Lodgepole Pine – -Biotechnology Progress, 23(5) 1130-1137
Berlin, A., Munoz, C., Gilkes, N., Massoumi Alamouti, S., Chung, P., Kang, K., Maximenko, V., Baeza, J., Freer, J., Mendoca, R. and Saddler, J.N. (2007). An Evaluation of British Columbian Beetle-Killed Hybrid Spruce for Bioethanol Productin – –Applied Biochemistry and Biotechnology, 136-140:267-280
Berlin, A., Maximenko, V., Gilkes, N. and Saddler, J.N. (2007). Optimization of Enzyme Complexes for Lignocellulose Hydrolysis – -Biotechnology and Bioengineering, 97(2):287-296
Ohgren, K., Bura, R., Lenicki, G., Saddler, J.N. and Zacchi, G. (2007). A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover – -Process Biochemistry, 42(5):834-839
Pan, X.-J., Xie, D., Yu, R., Lam, D. and Saddler, J.N. (2007). Pretreatment of lodgepole pine killed by mountain pine beetle using organosolv ethanol process: fractionation and process optimization – -Industrial & Engineering Chemistry Research, 46(8):2609-2617
Ohgren, K., Bura, R., Saddler, J.N. and Zacchi, G. (2006). Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. – -Bioresource Technology 93(13):2503-2510
Bjorheden, R., Domac, J., Mabee, W., Popescu, O., Richares, K., Richardson, J., Saddler, J.N., Smith, C.T. and Worgetter, M. (2006). Biofuels and Bioenergy: Challenges and Opportunities – -Proceedings of a joint workshop of IEA Bioenergy Task 29, Task 31 and Task 39, August 2006, Vancouver, BC, Canada
Pan, X Ehara K. Gilkes, N. Kadla, J. F. Saddler J. (2006). Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions and antioxidant activity – J. Agr. Food Chem. 54, 5806-5813, 2006.
Pan, X, Gilkes, N., Kadla, J., Pye, K., Saka, S., Ehara, K., Gregg, D., Xie, D., Lam, D. and Saddler, J. (2006). Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation Process: Optimization of process yields. – Biotechnology and Bioengineering, published online, DOI:10.1002/bit.20905
Berlin, A., Balakshin, M., Gilkes, N., Kadla, J., Maximenko, V, Kubo, S. and Saddler, J.N. (2006). Inhibition of cellulase, xylanase and ß-glucosidase activities by softwood lignin preparations – Journal of Biotechnology 125(2):198-209
Keating, J., Johansson, C., Saddler, J.N. and Beatson, R. (2006). The nature of chromophores in high extractive mechanical pulps: Western red cedar (Thuja plicata Donn) chemithermomechanical pulp (CTMP) – Holzforschung 60:365-371
Tu, M., Zhang, X., Kurabi, A., Gilkes, N., Mabee, W. and Saddler, J.N. (2006). Immobilization of ß-glucosidase on Eupergit C for lignocellulose hydrolysis – Biotechnology Letters. 28: 151-156
Pan, X.J., Gilkes, N. and Saddler, J. (2006). Effect of acetyl groups on enzymatic hydrolysis of cellulosic substates – -Holzforschung, 94, 851-861
Berlin, A., Maximenko, V., Bura, R., Kang, K., Gilkes, N. and Saddler, J. (2005). A rapid microassay to evaluate enzymatic hydrolysis of lignocellulosic subtrates – Wiley InterScience, published online, DOI: 10.1002/bit.20783
Berlin, A., Gilkes, N., Kurabi, A., Bura, R., Tu, M., Kilburn, D. and Saddler, J (2005). Weak lignin-binding enzymes – a novel approach to improve the activity of cellulases for hydrolysis of lignocellulosics – Applied Biochemistry and Biotechnology, 121-124:163-170
Mabee, W. and Saddler, J. (2005). Assessing the emerging biorefinery sector in Canada – Applied Biiochemestry and Biotechnology 121-124:765-778
Pan, X., Arato, C., Gilkes, N., Gregg, D.J., Mabee, W., Pye, E.K., Xiao, X. and Saddler, J.N. (2005). Biorefining of softwoods using ethanol organosolv pulping – preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. – Biotechnology and Bioengineering. Vol. 90
Pan, X., Xie, D., Gilkes, N., Gregg, D.J. and Saddler, J.N. (2005). Strategies to enhance the enzymatic hydrolysis of pretreated softwood with high residual lignin content – Applied Biochemistry and Biotechnology. Vol. 121-124
Alex Berlina, Neil Gilkesa, Douglas Kilburna, Renata Buraa, Alexander Markovb, Anton Skomarovskyb, Oleg Okunevc, Alexander Gusakovb, Vera Maximenkoa, David Gregga, Arkady Sinitsynb and John Saddler (2005). Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates – evidence for the role of accesory enzymes – – Enzyme and Microbial Technology Volume 37, Issue 2, 1 July 2005, Pages 175-184
Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Markov, A., Skomarovsky, A., Okunev, O., Gusakov, A., Maximenko, V., Gregg, D., Sinitsyn and Saddler, J.N. (2005). Evaluation of novel fungal cellulse preparations for ability to hydrolyze softwood substrates – evidence for the role of accessory enzymes – -Enzyme and Microbial Technology 37(2005) 175-184
Mabee, W.E., Gregg, D.J. and Saddler, J.N. (2004). International Energy Agency – Bioenergy. Current State of Fuel Ethanol Commercialization. Special Session B. – Applied Biochemistry and Biotechnology. Vol 113-116
Keating, J. D., Robinson, J., Cotta, M. A., Saddler, J. N., and Mansfield, S. D. (2004). An Ethanologenic Yeast Exhibiting Unusual Metabolism in the Fermentation of Lignocellulosic Hexose Sugars – Industrial Microbiology and Biotechnology. 31(5):235-244
Keating, J., Johansson, I., Saddler, J.N., and Beatson, R.P. (2004). Kinetics and Mechanism of Hydrogen Peroxide Brightening of Western Red Cedar CTMP – Paper being presented at PAPTAC (Pulp and Paper Technical Association of Canada) Conference in Parksville, BC on April 16 and 17, 2004
Saddler, J.N., Gregg, D.J. and Mabee, W.E. (2004). Progress in the Commercialization of Lignocellulosics-to-Ethanol – Paper being presented at the 2nd World Conference and Technology Exhibition on Biomass for Energy, Industry and Climate Protection, May 2004.
Pan, X., Zhang, X., Gregg, D.J., and Saddler, J.N. (2004). Enhanced enzymatic hydrolysis of steam exploded Douglas-Fir wood by alkali-oxygen post-treatment. – Applied Biochemistry and Biotechnology. Vol. 113-116. (Proceedings of the 25th Symposium on Biotechnology for Fuels and Chemicals, May 2003)
Xiao, Z., Zhang, X., Gregg, D.J. and Saddler, J.N. (2004). Effects of sugar inhibition on cellulase and B-glucosidase during enzymatic hydrolysis of softwood substrates. – Applied Biochemistry and Biotechnology. Vol 113 – 116.(Proceedings of the 25th Symposium on Biotechnology for Fuels and Chemicals, May 2003)
Keating, J.D., Robinson, J., Bothast, R.J., Saddler, J.N. and Mansfield, S.D. (2004). Characterization of a Unique Ethanologenic Yeast Capable of Fermenting Galactose. – Enzyme and Microbial Technology. 35(2/3):242-253
Cullis, I.F., Saddler, J.N. and Mansfield, S.D. (2004). Effect of initial moisture content and chip size on the bioconversion efficiency of softwood lignocellulosics. – Biotechnology and Bioengineering, 85(4):413-421.
Stebbing, D.W., Zhang, X., Soong, G., Mansfield, S.D. and Saddler, J.N. (2004). Fungal enzyme treatment of newsprint mill white water – impact on water and fiber properties. – Pulp and Paper Science, Vol. 30. No. 1:3-8
(2004). will second-generation ethanol be able to compete with first-generation ethanol?
(2004). Pan XJ, Saddler J.N. 2013. Effect of replacing Polyol by organosolv and kraft lignin on the property and structure of rigid polyurethane foam. Biotechnology for Biofuels 2013, 6:12
(2004). Panagiotopoulos, I.A., Chandra, R.P., Saddler, J.N. 2013. A two stage pretreatment approach to maximise sugar yield to enhance reactive lignin recovery from poplar wood chips. Bioresource Technology, 130, 570–577
O”Connor, D., Esteghlalian, A.R., Gregg, D.J. and Saddler, J.N. (2003). Carbon Balance of Ethanol from Wood: The effect of Feedstock Source in Canada. – The Role of Boreal Forests and Forestry in the Global Carbon Budget. pp. 289-296 (Proceedings of the International Science Conference, Edm. Alta. May 2000)
Bura, R., Bothast, R.J., Mansfield, S.D. and Saddler, J.N. (2003). Optimization of SO2-Catalysed Steam Pretreatment of Corn Fibre for Ethanol Production – Applied Biochemistry and Biotechnology, Vol. 105-108: 319-335.
Robinson, J., Keating, J.D., Mansfield, S.D. and Saddler, J.N. (2003). The fermentability of concentrated softwood derived hemicellulose fractions with and without supplemental cellulose hydrolysates. – Enzyme and Microbial Technology, 33(6), 757-765.
Mansfield, S.D. and Saddler, J.N. (2003). Application of Enzymes to Lignocellulosics – American Chemical Society – Oxford University Press
Graham, P.J., Gregg, D.J. and Saddler, J.N. (2003). Wood-Ethanol for Climate Change Mitigation in Canada – Applied Biochemistry and Biotechnology Vol. 105-108. (Proceedings of the 24th Symposium on Biotechnology for Fuels and Chemicals, May 2002)
Himmel, M.D., Baker, J.O. and Saddler, J.N., Editors (2003). Glycosyl Hydrolases for Biomass Conversion – ACS Symposium Series 769
Saddler, JN, RA Kozak and SB Watts (2002). A University Perspective on Innovations in Forestry Science and Technology. – The Forestry Chronicle 78(1):115-116.
Zhang, X., Eigendorf, G., Stebbing, D.W., Mansfield, S.D. and Saddler, J.N. (2002). Degradation of trilinolein by laccase enzymes. – Archives of Biochemistry and Biophysics. 405(1):44-54.
Esteghlalian, A.R., Mansfield, S.D. and Saddler, J.N. (2002). Cellulases: Agents for Fiber Modification or Bioconversion? The effect of substrate accessibility on cellulose enzymatic hydrolyzability. – L. Viikari & R. Lantto (Eds.), Biotechnology Progress, Vol. 21, pp. 21-36. Elsevier Science.
Esteghlalian, A., Graham, P., Gregg, D.J., O””Connor, D. and Saddler, J.N. (2002). The Potential of Short Rotation Forestry on Marginal Farmland in BC and Alberta to Provide a Feedstock for Energy Generation and to Reduce Greenhouse Gas Emissions – Suststainable Forest Management Network (Final project report 2002-5)
Mais, U., Esteghlalian, A.R. and Saddler, J.N. (2002). Influence of Mixing Regime on Enzymatic Saccharification of Steam-Exploded Softwood Chips. – Applied Biochemistry and Biotechnology, Vol 98-100, 463-472. (Proceedings of the 23rd Symposium on Biotechnology for Fuels and Chemicals, Colorado, May 2001)
Robinson, J., Keating, J., Boussaid, A., Mansfield, S.D. and Saddler, J.N. (2002). The influence of bark on the fermenation of douglas-fir whitewood pre-hydrolysates – Applied Microbiology and Biotechnology. 59 (4-5): 443-448
Bura, R., Mansfield, S.D., Saddler, J.N. and Bothast, R.J. (2002). SO2-catalysed steam explosion of corn fibre for ethanol production. – Applied Biochemistry and Biotechnology 98(100):59-72.
Lu, Y., Yang, B, Gregg, D.J., Saddler, J.N. and Mansfield, S.D. (2002). Cellulase adsorption and an evaluation of enzyme recycle during the hydrolysis of steam-exploded softwood residues. – Applied Biochemistry and Biotechnology, Vol. 98-100, 641-654.
Mais, U., Esteghlalian, A.R., Saddler, J.N. and Mansfield, S.D. (2002). Enhancing the enzymatic hydrolysis of cellulosic materials using simultaneous ball milling. – Applied Biochemistry and BiotechnologyVol 98-100, 815-832.
Yang, B., Boussaid, A., Mansfield, S.D., Gregg, D.J. and Saddler, J.N. (2002). A fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam exploded softwood substrates. – Biotechology and Bioengineering 77(6):678-684.
Zhang, X., Stebbing, D.W., Soong, G, Saddler, J.N. and Beatson, R.P. (2002). A combined fungal and enzyme treatment system to remove TMP/newsprint mill white waters substances. – TAPPI 1(2):21-27.
Boussaid, A., Cai Y., Robinson, J., Gregg, D.J., Nguyen, Q. and Saddler, J.N. (2001). Sugar recovery and fermentability of hemicellulose hydrolysates from steam-exploded softwoods containing bark. – Biotechnology Progress 17(5):887-892.
Ingesson, H., Zacchi, G., Yang, B., Esteghlalian, A.R. and Saddler, J.N. (2001). The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose. – Biotechnology 88:177-182.
Esteghlalian, A.R., Bilodeau, M., Mansfield, S.D.and Saddler, J.N.. (2001). Do enzymatic digestibility and Simons’ stain reflect the differences in the available surface area of lignocellulosic substrates. – Biotechnology Progress 17:1049-1054.
Esteghlalian, A.R., Srivastava, V., Gilkes, N.R., Kilburn, D.G., Warren, R.A.J. and Saddler, J.N. (2001). Do Cellulose Binding Domains Increase Substrate Accessibility? – Applied Biochemistry and Biotechnology, Vol. 91-93, 575-592 (Proceedings of the 22nd Symposium on Biotechnology for Fuels and Chemicals, Tenessee, May 2000)
S.M. Shevchenko, L. Pazner, J.N. Saddler (2001). Treatment of Organosolov and Steam Explosion Lignins with Molecular Hydrogen Iodide: A Structural Probe – Cellulose Chemistry and Technology. Vol 35, 3-4, 295-318
S.M. Shevchenko, K. Chang, D. G. Dick, D.J. Gregg and J.N. Saddler (2001). Structure and Properties of Lignin in Softwoods after SO2 – Catalyzed Steam Explosion and Enzymatic Hydrolysis – Cellulose Chemistry and Techonology. Vol. 35, 5-6, 487-502
Saddler, J.N., Zhang, X., Beatson, R.P., Stebbing, D.W., Cai, Y., Soong, JJ. (2001). Enzymatic and Microbial Treatment of Concentrated and Recycled Pulp Effluents – Sustainable Forest Management Network (Final project report 2001-21)
Johannsson, C.I., Saddler, J.N. and Beatson, R.P. (2000). Characterization of the Polyphenolics Related to the Colour of Western Red Cedar (Thuja plicata Donn) Heartwood – Holzforschung 54(3): 246-254
Johansson, I., Beatson, R.P. and Saddler, J.N. (2000). Fate and Influence of Western Red Cedar Extractivesin Mechanical Pulping. – Wood and Fibre Science 34(5): 389-401
Shevchenko, S., Paszner, L. and Saddler, J.N. (2000). Structural Changes in Lignin During Organosolv Pulping and Steam Explosion as Evidenced by Mild Depolymerization with Molecular Hydrogen Iodide. In press. – Cellulose Chemistry and Technology 35 (5/6)
Zhang, X., Stebbing, D.W., Saddler, J.N., Beatson, R.P. and Kruus, K. (2000). Enzyme Treatments of the Dissolved and Colloidial Substances Present in Mill White Water and the Effects on the Resulting Paper Properties. – Journal of Pulp and Paper Science. 3(20) pp. 321-335
Saddler, J.N. (2000). The Potential of Western Red Cedar as a Furnish for High Quality Mechanical Pulps. – Wood and Fibre Science
Esteghlalian, A., Boussaid, A., Gregg, D.J., Lee, K.H., Saddler, J.N., (2000). Steam Pretreatment of Douglas-Fir Wood Chips: Can Conditions for Optimum Hemicellulose Recovery Still Privide Adequate Access for Efficient Enzymatic Hydrolysis? – Applied Biochemistry and Biotechnology (Proceedings of the 21st Symposium on Biotechnology for Fuels and Chemicals, Colorado, May 1999)
Esteghlalian, A., Srivastava, V., Gilkes, N., Gregg, D.J.and Saddler, J.N. (2000). An Overview of Factors Influencing the Enzymatic Hydrolysis of Lignocellulosic Feedstocks. – ACS Series X, Ed. M. Himmel, J. Baker and J.N. Saddler.
Esteghlalian, A.R., Srivastava, V., Gilkes, N.R., Kilburn, D.G., Warren, R.A.J. and Saddler, J.N. (2000). Interaction Between Cellulose and a Cellulose-Binding Domain (CBDCex) From Bacterium Cellulomonas fimi and Its Effect on Substrate Enzymatic Susceptibility. – NREL Proceedings of the Twenty-second Symposium on Biotechnology for Fuels and Chemicals.
Shevchenko, S.M., Chang, K., Robinson, J. and Saddler, J.N. (2000). Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips. – Bioresource Technology 72(3): 207-211.
Serreqi, A., Gamboa, H., Stark, K., Saddler, J.N. and Breuil, C.. (2000). Resin acid markers for total resin acid content in-mill process lines of a TMP/CTMP pulp mill. – Water Research 34(5):1727-1733.
Wu, M.M., Chang, K., Gregg, D.J., Boussaid, A., Beatson, R. P. and Saddler, J.N. (1999). Optimization of steam explosion to enhance hemicellulose recovery and enzymatic hydrolysis of softwoods. – Applied Biochemistry and Biotechnology. Vol. 77-79: 47-54. (Proceedings of the 20th Symposium on Biotechnology for Fuels and Chemicals, Tennessee, May, 1998)
Zhang, X., Beatson, R.P., Cai, Y.J. and Saddler, J.N. (1999). Accumulation of specific dissolved and colloidal substances during white water recycling affects paper properties. – Pulp and Paper Science. Vol. 25(6):206-210.
Beatson, R.P., X. Zhang, D. Stebbing, and J. Saddler. (1999). The dissolved and colloidal fractions of white water: Impact of paper properties and degradation by enzymes. – -Vol. 1:200-203 in 10th Int’l Symp. on Wood and Pulp Chemistry, Yokohama, Japan.
Boussaid, A. and Saddler, J.N.. (1999). Adsorption and activity profiles of cellulases during the hydrolysis of two Douglas-fir pulps. – Enzyme and Microbiology Technology. 24 (3-4):138-143.
Boussaid, A., Robinson, J., Cai, Y.J., Gregg, D.J. and Saddler, J.N. (1999). Fermentability of the hemicellulose-derived sugars from steam-exploded softwood (Douglas-fir). – Biotechnology and Bioengineering 64(3): 284-289
Chandra, R.P., Beatson, R.P., de Jong, E. and Saddler, J.N. (1999). The Effects of Treatment with the White Rot Fungus Trametes Versicolor and Laccase Enzymes on the Brightness of Douglas-Fir Heartwood Derived Thermomechanical Pulps. – Wood Chemistry and Technology. 19(1-2): 61-78.
Lumme, M.S., Mansfield, S.D. and Saddler, J.N. (1999). The effects of hydrolytic enzyme treatments on three British Columbian interior fir kraft pulps differing in their initial fiber coarseness. – Wood and Fiber Science. 31(4): 385-393.
Mansfield, S.D., Mooney, C. and Saddler, J.N. (1999). Substrate and enzyme characteristics that limit cellulose hydrolysis. – Biotechnology Progress. 15(5): 804-816.
Ramos, L.P., Filho, A.Z., Deschamps, F.C. and Saddler, J.N.. (1999). The effect of trichoderma cellulases on the fine structure of a bleached softwood kraft pulp. – Enzyme and Microbiology Technology. 24:371-380.
Saddler, J.N., X. Zhang, D. Stebbing, R.P. Beatson, B.G. Chalmers, and J.T. Ling. (1999). The different effects of white water dissolved and colloidal fractions on paper properties and effects of enzyme treatments on their removal. – PACWEST, Whistler. pp. 18-24.
Shevchenko, S.M., Beatson, R.P. and Saddler, J.N. (1999). The nature of lignin from steam explosion/enzymatic hydrolysis of softwood: Structural features and possible uses. – Applied Biochemistry and Biotechnology. Vol. 77-79: 867-876. (Proceedings of the 20th Symposium on Biotechnology for Fuels and Chemicals, Tennessee, May 1998)
Mansfield, S.D. and Saddler, J.N. (1999). Sheet properties of Douglas-fir kraft pulp after selective treatments of different fibre length fractions with cellulases. – Pulp and Paper Science 25(3): 84-89.
Mansfield, S.D., Swanson, D.J., Roberts, N., Olson, J.A. and Saddler, J.N. (1999). Enhancing Douglas-fir pulp properties with a combination of enzyme treatments and fiber fractionation. – Tappi. 82(5):152-158.
Mooney, C.A., Mansfield, S.D., Beatson, R.P. and Saddler, J.N. (1999). The effect of fiber characteristics on hydrolysis and cellulase accessibility to softwood substrates. – Enzyme Microbilogy Technology. 25(8-9): 644-650.
Gübitz, G.M., C.D. Edgar, S.D. Mansfield, J.N. Saddler, and W. Steiner. (1999). Role of cellulase/hemicellulase synergism in the modification of pretreated wood substrates. – Int’l Symp. Biotechnol. Lignocell. Mat. Curitiba, Brazil. pp. 45-56.
Lee, D., A.H.C. Yu and J.N. Saddler. 1996 (1999). Effect of lignin on cellulose recycling strategies. – pp. 547-550 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int’l Conf. on Biotechnology in the Pulp and Paper Industry.
Gübitz, G.M., S.D. Mansfield and J.N. Saddler. (1998). Effectiveness of two endoglucanases from Gloeophyllum sp. in deinking mixed office waste. – 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:135-138.
Lumme, M.S., S.D. Mansfield, and J.N. Saddler. (1998). Effect of enzyme treatment on two British Columbia interior fir kraft pulps. – 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:163-166.
Mansfield, S.D., A.R. Dickson, and J.N. Saddler. (1998). Improving paper properties by a selective enzymatic treatment of coarse pulp fibres. – 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 1:189-192.
Mansfield, S.D., B. Saake, G.M. Gübitz, E. de Jong, J. Puls, and J.N. Saddler. (1998). Identification, purification and characterisation of the predominant endoglucanases from two brown-rot fungal strains of Gloeophytllum. – pp. 227-244 in Proc. 1997 Tricel Symp. Ghent, Belgium.
Mooney, C.A., S.D. Mansfield, R.P. Beatson, and J.N. Saddler. (1998). The effect of fibre characteristics on accessibility and hydrolysis of softwood substrates. – 7th International conference on Biotechnology in the Pulp and Paper Industry. June 16-19, 1998. Vancouver, BC. CPPA 3:99-102.
Mooney, C.A., S.D. Mansfield, M.G. Touhy, and J.N. Saddler. (1998). The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods. – Bioresource Technol. 64(2):113-119.
Stalbrand, H., S.D. Mansfield, J.N. Saddler, D.G. Kilburn, R. Anthony J. Warren, and N.R. Gilkes. (1998). Analysis of molecular size distributions of cellulose during hydrolysis of cellulose by recombinent cellulomonas fimi Beta-1, 4-glucanases. – App. Environ. Microbiol. 64(7):2374-2379.
Edgar, C.D., S.D Mansfield, G.M. Gübitz, and J.N. Saddler. (1998). The synergistic effects of endoglucanase and xylanase in modifying Douglas-fir kraft pulp. Oxford University Press. pp.75-87 in Eriksson, K.-E. L and Cavaco-Paulo, A.M. (eds.), Enzyme Applications in Fibre Processing.
Gregg, D.J., A. Boussaid and J.N. Saddler. (1998). Technoeconomic evaluations of a generic wood-to-ethanol process: Process effect of increased cellulose yields and enzyme recycle. – Bioresource Technol. 63(1998):7-12.
Gubitz, G.M., D.W. Stebbing, C.I. Johansson, and J.N. Saddler. (1998). Lignin-hemicellulose-complexes restrict enzymatic solubilisation of mannan and xylan from dissolving pulp. – Appl. Microbiol. Biotechnol. 50:390-395.
Gubitz, G.M., S.D. Mansfield, D. Bohm, and J.N. Saddler. (1998). Effect of endoglucanases and hemicellulases in magnetic and flotation deinking of xerographic and laser-printed papers. – Journal of Biotechnology 65:209-215.
Kyriacou, P., E. de Jong, C. Johansson, R.P. Chandra, and J.N. Saddler. (1998). Bleaching of western red cedar and Douglas-fir mechanical pulps. – TAPPI J. 81(6):188-195.
Mansfield, S.D., J.N. Saddler and G.M. Gubitz. (1998). Characterisation of two endoglucanases from the brown-rot fungi Gloeophyllum sepiarium and Gloeophyllum trabeum. – Enzyme Microb. Technol. 23:133-140.
Ryan, S.E., E. de Jong, G.M. Gubitz, M. Tuohy, and J.N. Saddler. (1998). The potential of hydrolytic enzymes to modify Douglas-fir derived bleached mechanical pulps. The Royal Society of Chemistry. pp. 214-226 in Claeyssens, M. et al. (eds.), Carbohydrases from T. reesei and other microorganisms.
Gregg, D.J. and J.N. Saddler. . (1997). A review of techno-economic modelling methodology for a wood-to-ethanol process. – In Proc. 18th Symp. Biotechnology for Fuels and Chemicals. Appl. Biochem. Biotechnol. 63/65:609-623
Liss, S.N., P.A. Bicho, P.N. McFarlane, and J.N. Saddler. (1997). Microbiology and biodegradation of resin acids in pulp mill effluents: A mini review. – Can. J. Microbiol. 75:599-611.
Wong, K.K.Y., L.A. Martin, F.M. Gama, J.N. Saddler, and E. de Jong. (1997). Bleach boosting and direct brightening by multiple xylanase treatments during peroxide bleaching of Kraft pulps. – Biotechnol. Bioengin. 54:312-318.
de Jong, E., K.K.Y. Wong, and J.N. Saddler. (1997). The mechanism of xylanase prebleaching of kraft pulp – an examination using model pulps prepared by depositing lignin and xylan on cellulose fibers. – Holzforschung 51:19-26.
Zhang, Y., P.A. Bicho, C. Breuil, J.N. Saddler, and S.N. Liss. (1997). Resin acid degradation by bacterial strains grown on CTMP effluent. – Water Sci. Tech. 35:33-39.
Gregg, D.J. and J.N. Saddler. (1997). The IEA network: Biotechnology for the conversion of lignocellulosics. – pp. 55-60 in Proc. 2nd European Motor Biofuels Forum, Graz, Austria.
Saddler, J.N. and D.J. Gregg. (1997). Steam explosion and bioconversion of softwoods. – pp. 135-138 in M. Stumborg (ed.), Proc. ’97 Ethanol Research and Development Workshop, Ottawa.
Mansfield, S.D. and J.N. Saddler. (1997). The use of enzymes to enhance Douglas-fir pulp characteristics. – 1997 TAPPI Biological Sciences Symposium. October 19-23, 1997. San Francisco, CA. TAPPI Press 1:279-282.
Mansfield, S.D., E. de Jong and J.N. Saddler. (1997). Cellobiose dehydrogenase, an active agent in cellulose depolymerization. – App. Environ. Microbiol. 63(10): 3804-3809.
Mansfield, S.D., E. de Jong and J.N. Saddler. (1997). The potential of enzymes to selectively modify pulp characteristics. – In Proc. ’97 Spring Conf. of CPPA Pacific Coast and Western Branches, Whistler Paper 3B:1-4.
Mansfield, S.D., E. de Jong, R.S. Stephens, and J.N. Saddler. (1997). Physical characterization of enzymatically modified kraft pulp fibres. – J. Biotechnol. 57(1-3): 205-216.
Mooney, C.A., S.D. Mansfield, M.G. Touhy, and J.N. Saddler. (1997). The effect of lignin content on cellulose accessibility and enzymatic hydrolysis of softwood pulps. – 1997 TAPPI Biological Sciences Symposium. October 19-23, 1997. San Francisco, CA. TAPPI Press 1:259-265.
Li, K., A. Serreqi, C. Breuil and J.N. Saddler. (1997). Quantification of resin acids in CTMP effluents using an enzyme-linked immunosorbent assay. – Water Science & Technol. 35(2-3):93-99.
Mansfield, S.D., K.K.Y. Wong, E. de Jong, and J.N. Saddler. (1996). Xylanase prebleaching of different fiber length fractions of Douglas-fir kraft pulp. – Appl. Microbiol. & Biotechnol. 46:319-326.
de Jong, E., K.K.Y. Wong, L.A. Martin, S.D. Mansfield, F.M. Gama, and J.N. Saddler. (1996). Molecular mass distribution of materials solubilized by xylanase treatment of Douglas-fir kraft pulp. American Chemical Society (ACS) Symp. Series No. 655. pp. 44-62 in T. Jeffries (ed.), Enzymes for Pulp and Paper Processing.
Mansfield, S.D., K.K.Y. Wong, T. Astle, and J.N. Saddler. (1996). Enzymatic modification of coarse pulp fibers derived from Douglas-fir. – pp. 173-176 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int’l Conf. on Biotechnology in the Pulp and Paper Industry.
Mansfield, S.D., K.K.Y. Wong, E. de Jong, and J.N. Saddler. (1996). Modification of Douglas-fir mechanical and kraft pulps by enzymatic treatment. – Tappi J. 79(8):125-132.
Werker, A.G., P.A. Bicho, J.N. Saddler, and E.R. Hall. (1996). Surface tension changes related to the biotransformation of dehydro-abietic acid by Mortierella isabellina. – pp. 139-150 in M.R. Servos (ed.), Environmental Fate and Effects of Pulp and Paper Mill Effluents. St. Lucie Press.
Wong, K.K.Y., E. de Jong, and J.N. Saddler. (1996). Size-exclusion chromatography of lignin- and carbohydrate-containing samples using alkaline effluents. – J. Chromatography A 737:193-203.
Wong, K.K.Y., E. de Jong, and J.N. Saddler. (1996). Lignin-xylan complexes in kraft pulp appear to be target substrates for xylanase prebleaching. Proc. 6th Int’l Conf. on Biotechnology in the Pulp and Paper Industry. pp. 93-98 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research.
Wong, K.K.Y., S.L. Nelson, and J.N. Saddler. (1996). Xylanase treatment for the peroxide bleaching of oxygen delignified kraft pulps derived from three softwood species. – J. Biotechnol. 48:137-145.
Wong, K.K.Y., S. Yokota, J.N. Saddler, and E. de Jong. (1996). Enzymatic hydrolysis of lignin-carbohydrate complexes isolated from kraft pulps. – J. Wood Chem. Technol. 16(2): 121-138.
Bicho, P.A., T. Chen, S. Avramidis, R.L. Zwick, C. Breuil, and J.N. Saddler. (1996). Characterization and treatment of condensates generated from radio-frequency/vacuum dried softwoods. – For. Prod. J. 46(10):51-56.
Li, K., T. Chen, P. Bicho, C. Breuil, and J.N. Saddler. (1996). Factors affecting gas chromatographic analysis of resin acids present in pulp mill effluents. – Toxicological and Environm. Chem. 57:1-16.
Li, K., T. Chen, P. Bicho, C. Breuil, and J.N. Saddler. (1996). A comparison of gas chromatographic and inununochemical methods for quantifying resin acids. St. Lucie Press. pp. 119-128 in Servos el al. (eds.), Environmental Fate and Effects of Pulp and Paper Mill Effluents.
de Jong, E., K.K.Y., L.R. Windsor, and J.N. Saddler. (1996). Xylanase prebleaching of model pulp. Proc. 6th Int’l Conf. on Biotechnology in the Pulp and Paper Industry. pp. 127-130 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research.
Gregg, D. and J.N. Saddler. (1996). Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood-to-ethanol process. – Biotech/Bioeng. 51:375-383.
Gregg, D. and J.N. Saddler. (1996). A technoeconomic assessment of the pretreatment and fractionation steps of a biomass-to-ethanol process. – Appl. Biochem. Biotechnol. 57/58:711-727.
Bicho, P.A., V.A. Martin and J.N. Saddler. (1996). Resin acid biodegradation by bacteria isolated from bleached kraft mill effluent: Specificity and induction. – pp. 611-616 in E. Srebotnik and K. Messner (eds.), Advances in Applied and Fundamental Research. Proc. 6th Int’l Conf. on Biotechnology in the Pulp and Paper Industry.
Bullock, C.M., P.A. Bicho, and J.N. Saddler. (1996). The influence of the high and low molecular weight fractions on the microbial activity of activated sludge. – Water Research 30(5):1095-1102.
Bullock, C.M., P.A. Bicho, Y. Zhang, and J.N. Saddler. (1996). A solid chemical oxygen demand (COD) method for determining biomass in waste waters. – Water Research 30(5):1280-1284.
Nelson, S.N., K.K.Y. Wong, R.P. Beatson, and J.N. Saddler. (1995). The use of xylanase for peroxide bleaching of kraft pulps derived from different softwood species. – Pulp and Paper Canada 96(7):42-45.
Nelson, S., K.K.Y. Wong, R.P. Beatson, and J.N. Saddler. (1995). Xylanase prebleaching of brownstock and oxygen delignified kraft pulp derived from Douglas-fir. – pp. 413-418 in Proc. IWC Meeting, Helsinki.
Werker, A.G., P.A. Bicho, J.N. Saddler, and E.R. Hall. (1995). Surface tension changes related to the biotransformation of dehydroabietic acid by Mortierella isabellina. – pp. 134-137 in Servos et al. (eds.), Environmental Fate and Effects of Pulp and Paper Mill Effluents. St. Lucie Press.
Yokotu, S., K.K.Y. Wong, J.N. Saddler, and I.D. Reid. (1995). Molecular weight distribution of xylan/lignin mixtures from kraft pulps. – Pulp & Paper Canada 96(4):39-41.
Yu, A.H.C. and J.N. Saddler (1995). Identification of essential cellulase components in the hydrolysis of a steam-exploded substrate – Biotechnol. Appl. Biochem. 21:185-202.
Yu, A.H.C., D. Lee and J.N. Saddler (1995). Adsorption and desorption of cellulase components during hydrolysis of a steamexploded birch substrate. – Biotechnol. Appl. Biochem. 21:203-216.
Bicho, P.A., V. Martin, and J.N. Saddler. (1995). Growth, induction and substrate specificity of dehydroabietic acid degrading bacteria isolated from a kraft mill effluent enrichment. – Appl. Environ. Microbiol. 61(9):3245-4350.
Gregg, D. and J.N. Saddler. (1995). Bioconversion of lignocellulosic residues to ethanol: process flowsheet development. – Biomass and Bioenergy 9:287-302.
Li, K., M. Chester, J. Kutney, C. Breuil, and J.N. Saddler. (1995). Development of an immunoassay for the detection of resin acids in pulp mill effluents. Kluwer Academic Publishers. pp. 675-688 in M. Moo-Young, W.A. Anderson, A.M. Chakrabarty (eds.), Environmental Biotechnology: Principles and Practice.
Saddler, J.N. and Penner, M.H. Editors (1995). Enzymatic Degradation on Insoluble Carbohydrates – ACS Symposium Series 618. (Proceedings from the 207th National Meeting of the American Chemical Society, California, March 1994)